A new low-pressure plasma-based approach to activate the surface of BN nanoparticles (BNNPs) in order to facilitate the attachment of folate acid (FA) molecules for cancer-specific therapy is described.Plasma treatment of BNNPs (BNNPsPT) was performed in a radiofrequency plasma reactor using ethylene and carbon dioxide monomers.The carboxyl groups deposited on the surface of BNNPsPT were activated by baseball scoreboards for sale N,N’-dicyclohexylcarbodiimide (DCC) and participated in the condensation reaction with ethylene diamine (EDA) to form a thin amino-containing layer (EDA-BNNPPT).
Then, the DCC-activated FA was covalently bonded with BNNPsPT by a chemical reaction between amino groups of EDA-BNNPsPT and carboxyl groups of FA.Density functional theory calculations showed that the pre-activation of simply boho classroom FA by DCC is required for grafting of the FA to the EDA-BNNPsPT.It was also demonstrated that after FA immobilization, the electronic characteristics of the pteridine ring remain unchanged, indicating that the targeting properties of the FA/EDA-BNNPsPT nanohybrids are preserved.